[HTML][HTML] Superoxide-induced stimulation of protein kinase C via thiol modification and modulation of zinc content
LT Knapp, E Klann - Journal of Biological Chemistry, 2000 - ASBMB
We investigated the effects of mild oxidation on protein kinase C (PKC) using the
xanthine/xanthine oxidase system of generating superoxide. Exposure of various PKC
preparations to superoxide stimulated the autonomous activity of PKC. Similarly, thiol
oxidation increased autonomous PKC activity, consistent with the notion that superoxide
stimulates PKC via thiol oxidation. The superoxide-induced stimulation of PKC activity was
partially reversed by reducing agents, suggesting that disulfide bond formation contributed …
xanthine/xanthine oxidase system of generating superoxide. Exposure of various PKC
preparations to superoxide stimulated the autonomous activity of PKC. Similarly, thiol
oxidation increased autonomous PKC activity, consistent with the notion that superoxide
stimulates PKC via thiol oxidation. The superoxide-induced stimulation of PKC activity was
partially reversed by reducing agents, suggesting that disulfide bond formation contributed …