[HTML][HTML] Mutations in the double-stranded RNA-activated protein kinase insert region that uncouple catalysis from eIF2α binding
R Cai, BRG Williams - Journal of Biological Chemistry, 1998 - ASBMB
The interferon-induced, double-stranded RNA (dsRNA)-activated protein kinase, PKR,
inhibits protein synthesis via phosphorylation of the alpha subunit of the translation initiation
factor eIF2. A kinase insert region N-terminal of PKR kinase subdomain V, which is
conserved among eIF2α kinases, has been proposed to determine substrate specificity of
these kinases. To investigate the function of this kinase insert region, selective PKR mutants
were generated, and kinase activities and eIF2α affinities were analyzedin vitro. The in vivo …
inhibits protein synthesis via phosphorylation of the alpha subunit of the translation initiation
factor eIF2. A kinase insert region N-terminal of PKR kinase subdomain V, which is
conserved among eIF2α kinases, has been proposed to determine substrate specificity of
these kinases. To investigate the function of this kinase insert region, selective PKR mutants
were generated, and kinase activities and eIF2α affinities were analyzedin vitro. The in vivo …
