Stoichiometry of the reaction of oxyhemoglobin with nitrite
H Kosaka, K Imaizumi, K Imai, I Tyuma - Biochimica et Biophysica Acta …, 1979 - Elsevier
During the reaction of oxyhemoglobin (HbO 2) with nitrite, the concentration of residual
nitrite, nitrate, oxygen, and methemoglobin (Hb+) was determined successively. The results
obtained at various pH values indicate the following stoichiometry for the overall reaction:
4HbO 2+ 4NO− 2+→ 4Hb++ 4NO− 3+ O 2+ 2H 2 O (Hb denotes hemoglobin monomer). NO
2− binds with methemoglobin noncooperatively with a binding constant of 340 M− 1 at pH
7.4 and 25° C. Thus, the major part of Hb+ produced is aquomethemoglobin, not …
nitrite, nitrate, oxygen, and methemoglobin (Hb+) was determined successively. The results
obtained at various pH values indicate the following stoichiometry for the overall reaction:
4HbO 2+ 4NO− 2+→ 4Hb++ 4NO− 3+ O 2+ 2H 2 O (Hb denotes hemoglobin monomer). NO
2− binds with methemoglobin noncooperatively with a binding constant of 340 M− 1 at pH
7.4 and 25° C. Thus, the major part of Hb+ produced is aquomethemoglobin, not …
