[HTML][HTML] Overall sulfation of heparan sulfate from pancreatic islet β-TC3 cells increases maximal fibril formation but does not determine binding to the amyloidogenic …
RL Hull, MJ Peters, SP Perigo, CK Chan… - Journal of Biological …, 2012 - ASBMB
Islet amyloid, a pathologic feature of type 2 diabetes, contains the islet β-cell peptide islet
amyloid polypeptide (IAPP) as its unique amyloidogenic component. Islet amyloid also
contains heparan sulfate proteoglycans (HSPGs) that may contribute to amyloid formation by
binding IAPP via their heparan sulfate (HS) chains. We hypothesized that β-cells produce
HS that bind IAPP via regions of highly sulfated disaccharides. Unexpectedly, HS from the β-
cell line β-TC3 contained fewer regions of highly sulfated disaccharides compared with …
amyloid polypeptide (IAPP) as its unique amyloidogenic component. Islet amyloid also
contains heparan sulfate proteoglycans (HSPGs) that may contribute to amyloid formation by
binding IAPP via their heparan sulfate (HS) chains. We hypothesized that β-cells produce
HS that bind IAPP via regions of highly sulfated disaccharides. Unexpectedly, HS from the β-
cell line β-TC3 contained fewer regions of highly sulfated disaccharides compared with …
