The microenvironment of the thymus consists of functionally discrete niches composed of
distinct stromal cell subsets. Clinically relevant changes affecting T-cell differentiation occur
within these niches with age and injury caused by irradiation and chemotherapy treatments.
The study of thymic stromal cells has been hampered by the technical difficulty in isolating
significant numbers of this important population. Here we present an improved protocol for
enzymatic isolation of stromal cells that enables comparative flow cytometric analyses and …