Estrogen enhances immunoglobulin production by human PBMCs
N Kanda, K Tamaki - Journal of Allergy and Clinical Immunology, 1999 - Elsevier
Background: It has been suggested that estrogen may enhance humoral immune responses
and may be involved in the pathogenesis of autoimmune diseases. Objective: We studied
the in vitro effects of 17β-estradiol (E2) on spontaneous immunoglobulin production by
human PBMCs. Methods: PBMCs from healthy human volunteers were cultured with E2.
Levels of IgG and IgM and cytokine activity were measured by ELISA. Proliferation was
determined by [3H]-thymidine uptake. The cell viability was assessed by a trypan blue …
and may be involved in the pathogenesis of autoimmune diseases. Objective: We studied
the in vitro effects of 17β-estradiol (E2) on spontaneous immunoglobulin production by
human PBMCs. Methods: PBMCs from healthy human volunteers were cultured with E2.
Levels of IgG and IgM and cytokine activity were measured by ELISA. Proliferation was
determined by [3H]-thymidine uptake. The cell viability was assessed by a trypan blue …