Fluorogenic phospholipid substrate to detect lysophospholipase D/autotaxin activity
CG Ferguson, CS Bigman, RD Richardson… - Organic …, 2006 - ACS Publications
CG Ferguson, CS Bigman, RD Richardson, LA van Meeteren, WH Moolenaar, GD Prestwich
Organic letters, 2006•ACS PublicationsLysophospholipase D (lysoPLD), also known as autotaxin (ATX), is an important source of
the potent mitogen lysophosphatidic acid (LPA). Two fluorogenic substrate analogues for
lysoPLD were synthesized in nine steps from (S)-PMB-glycerol. The substrates (FS-2 and FS-
3) show significant increases in fluorescence when treated with recombinant ATX and have
potential applications in screening for this emerging drug target.
the potent mitogen lysophosphatidic acid (LPA). Two fluorogenic substrate analogues for
lysoPLD were synthesized in nine steps from (S)-PMB-glycerol. The substrates (FS-2 and FS-
3) show significant increases in fluorescence when treated with recombinant ATX and have
potential applications in screening for this emerging drug target.
Lysophospholipase D (lysoPLD), also known as autotaxin (ATX), is an important source of the potent mitogen lysophosphatidic acid (LPA). Two fluorogenic substrate analogues for lysoPLD were synthesized in nine steps from (S)-PMB-glycerol. The substrates (FS-2 and FS-3) show significant increases in fluorescence when treated with recombinant ATX and have potential applications in screening for this emerging drug target.
ACS Publications